Biotechnology

Actinomycetes

Actinomycetes belong to the group of prokaryotic, gram-positive bacteria which are having a filamentous structure. Its filamentous structure resembles the fungal mycelium, which consists of a highly dense and filamentous network. Due to the filamentous structure of Actinomycetes, it also refers as “Thread or Ray bacteria”. The cell wall and the internal structure of the …

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Restriction Enzyme

Restriction enzyme refers to as “Restriction endonuclease” which was discovered during the study of Entero-bacteriophage where the E.coli inhibits the phage activity. In 1978, Werner Arber, Daniel Nathans, Hamilton O Smith won the Nobel Prize for the characterization and discovery of restriction enzyme. Restriction endonuclease also refers to as “Molecular scissors” which are widely used …

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Colony Hybridization

Introduction: Colony hybridization is the method which was first given by the scientists Grinstein and Hogness in the year 1975. This method isolates the specific DNA sequences or genes from the hybrid DNA. Colony hybridization is carried out by the nitrocellulose filter paper. This technique is very much similar to the replica plating. Colony hybridization …

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Biohydrogen

Introduction: Biohydrogen is now being commercially produced as a “Biofuel”. It is the advanced biofuel, which makes the use of living biomass or organisms for its production. Biohydrogen is now considered best among all the biofuels as it accounts to fulfil all the energy demands as it can obtain from the sustainable resources like: Biological …

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Meristem Culture

Meristem culture particularly involves the cultivation of the shoot apical meristem. This technique also refers to Shoot tip or Apical meristem culture. In 1952 Morel and Martin were the first scientists who developed the technique of meristem culture for in-vivo virus eradication of Dahlia. Orchid cymbidium was micropropagated using this culture by Morel in 1965. …

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