Restriction enzyme refers to as “Restriction endonuclease” which was discovered during the study of Entero-bacteriophage where the E.coli inhibits the phage activity. In 1978, Werner Arber, Daniel Nathans, Hamilton O Smith won the Nobel Prize for the characterization and discovery of restriction enzyme. Restriction endonuclease also refers to as “Molecular scissors” which are widely used …
Dot blot technique is a method of identifying the presence of DNA, RNA and Protein in the sample. It is the simplified form of Southern, Northern and Western blotting for the isolation of DNA, RNA and protein respectively. This method can detect the presence or absence of biomolecule in a single run. Dot blot technique …
Southern Hybridization is the technique which was first given by the scientist E. M. Southern in 1975. It is a type of blotting method, which involves a transfer of the DNA from the solid agarose gel to the adsorbent medium like nitrocellulose or nylon filter paper. Southern blotting is done after the process of DNA …
Colony hybridization is the method which was first given by the scientists Grinstein and Hogness in the year 1975. This method isolates the specific DNA sequences or genes from the hybrid DNA. Colony hybridization is carried out by the nitrocellulose filter paper. This technique is very much similar to the replica plating. Colony hybridization involves …
Biohydrogen is now being commercially produced as a “Biofuel”. It is the advanced biofuel, which makes the use of living biomass or organisms for its production. Biohydrogen is now considered best among all the biofuels as it accounts to fulfil all the energy demands as it can obtain from the sustainable resources like: Biological splitting …
Animal cell culture is one of the major tools now in the field of life science. It is the in-vitro technique where the cells are grown in the laboratory conditions by providing a proper nutrient source, growth factors and the environmental factors for the cell growth and division. In the Animal cell culture, the cells …
Meristem culture particularly involves the cultivation of the shoot apical meristem. This technique also refers to Shoot tip or Apical meristem culture. In 1952 Morel and Martin were the first scientists who developed the technique of meristem culture for in-vivo virus eradication of Dahlia. Orchid cymbidium was micropropagated using this culture by Morel in 1965. …
Ovary culture is the in-vitro technique which is carried outside on a suitable nutrient medium in an unpollinated flower to grow new haploid plants. The process of ovary culture refers as “Gynogenesis” as the ovary is the female reproductive part or also refers to as “Gynoecium”. Gynogenesis was first studied by the scientist San Noem in …
Anther culture is a type of tissue culture technique, where a part of a plant, i.e. Anther gives rise to an androgenic haploid plant when cultured in a nutrient medium under optimal conditions that are required for the growth of the cell. The process of anther culture comes under the process of “Androgenesis” which merely …
The difference between Endonuclease and Exonuclease is mainly due to the following two properties like mode of action and the site of cleavage. Mode of action: Endo-nuclease cuts the nucleotide bases from the middle by breaking the phosphodiester backbone of nucleotide. Exo-nuclease cuts the nucleotide bases at the ends by breaking the phosphodiester backbone of …