A restriction enzyme is produced within the bacterial cell due to which it is also called “Restriction endonuclease”. In 1978, Werner Arber, Daniel Nathans, Hamilton O Smith won the Nobel Prize for the characterization and discovery of restriction enzyme during the study of Entero-bacteriophage. According to their study, the bacteria possess some restriction activity that …
Dot blot technique is a method of identifying DNA, RNA and Protein in the sample. It is the simplified form of Southern, Northern and Western blotting for DNA, RNA, and protein isolation, respectively. This method can detect the presence or absence of biomolecule in a single run. Dot blot technique is a prevalent method in …
Southern hybridization is the technique, first given by the scientist E. M. Southern in 1975. It is a type of blotting method that involves transferring the DNA from the solid agarose gel to the adsorbent medium like nitrocellulose or nylon filter paper. Southern blotting is done after DNA hybridization. The target DNA is first cleaved …
Colony hybridization is the method first given by the scientists Grinstein and Hogness in the year 1975. This method isolates the specific DNA sequences or genes from the hybrid DNA. Colony hybridization makes the use of the nitrocellulose filter paper. This technique is very much similar to the replica plating. Colony hybridization involves replicas preparation …
Biohydrogen is now commercially produced as a biofuel. It is the advanced biofuel, which makes the use of living biomass or organisms for the production. Biohydrogen is now considered best among all the biofuels as it accounts to fulfil all the energy demands as it can be obtained from the sustainable resources like: Biological splitting …
Animal cell culture is one of the important tools now in the field of life science. It is the in-vitro technique, in which the cells are grown in the laboratory conditions under proper nutrient source, growth factors and the controlled environmental conditions for the cell growth and division. The cells in the animal cell culture …
Meristem culture particularly involves the cultivation of the shoot apical meristem. This technique is also called a shoot tip or apical meristem culture. It was reported in the year 1952 by the scientist’s named Morel and Martin. Orchid cymbidium was micropropagated using this culture by Morel in 1965. The meristem culture is very much similar …
Ovary culture is the in-vitro technique carried outside, i.e. on a suitable nutrient medium to grow new haploid plants by taking an unpollinated flower. The ovary culture process is called gynogenesis because an ovary is a female reproductive part called gynoecium. Gynogenesis was first studied by a scientist named San Noem in the year 1976. For …
Anther culture is a type of tissue culture technique, where a part of a plant (anther) gives rise to an androgenic haploid plant when cultured on a nutrient medium under optimal conditions. The nutrient media provide all the necessary factors that are required for the growth of the cell. The anther culture technique comes under …
The difference between endonuclease and exonuclease is mainly due to the two properties like mode of action and the site of cleavage. Mode of action: Endo-nuclease cuts the nucleotide bases from the middle by breaking the phosphodiester backbone of nucleotide. Exo-nuclease cuts the nucleotide bases at the ends by breaking the phosphodiester backbone of nucleotide …