The mechanism of enzyme action depends upon the two factors, namely enzyme’s specificity and transition state of the reactants or substrates. The enzyme’s specificity is due to its active site, which seems like a small aperture or opening. The enzyme’s active site allows specific binding of an enzyme with the substrate due to residues like …
Physical mutagenesis is a mutation in the cells, tissues and DNA of the organisms induced by physical mutagenic agents like radiation and heat. Thus, mutagenesis (muta: means changes and genesis: means origin or outset) is a phenomenon defined as the origination of any changes that can alter the gene sequence and structure, thereby causing mutation. …
Air sampling methods make the use of impaction, impingement and centrifugal collection principle to entrap the contaminants from the known volume of the air sample. The volume of collected air is first measured, and then it is expressed in the concentration against the volume of contaminated air, either in ppm (parts per million) or (mg/m3). …
Hot air oven sterilization is a process of decontamination carried out by an electrical device that utilizes dry heat to sterilize various type of equipment or articles kept inside its chamber. It is used for sterilizing objects that cannot be subjected to wet sterilization and objects that are less prone to dry heat. It was …
Hanging drop method permits the examination of the living microorganisms, which involves fixation of microbial suspension into a drop of fluid over the glass slide. Therefore hanging drop method is the process, in which the microorganisms are suspended into a drop of fluid. It is a modified version of the wet mount technique. The shape, …
Food preservation techniques have led to an increase in the longevity or shelf life of food items by introducing different ways of preserving methods. Its study is not only important in the field of food science, but also in daily lives, to set up food businesses etc. The methods of food preservation include some inexpensive …
Trickling filter process is one of the types of aerobic wastewater treatment. It is a fixed-bed bioreactor that is the part of secondary wastewater treatment, which eliminates the coarse particles, suspended organic and inorganic waste, small colloids etc. out of the primary effluent. A trickling filter is also called biological filter, as it makes the …
Activated sludge treatment can define as a conventional method, which can separate the solid wastes, suspended organic matter, soluble matter and parasites. It is a biological system that makes the use of living bacterial flocs to degrade the organic matter of the sewage and industrial waste in aerated bioreactors. Activated sludge treatment involves a series …
Difference between absorbent and adsorbent is mainly due to the following factors like their mechanism and the phenomena they follow: Mechanism: The mechanism of absorbent and absorbent is to retain the particles or molecules, in which a former absorbs the particles into the matrix, and the latter adsorbs the particles over the solid matrix. Phenomena: …
Prokaryotic ribosomes are dense structures, which solely contain RNA and proteins. The ribosomes in the prokaryotic cell are thoroughly distributed in the cell cytosol. There are two subunits of prokaryotic ribosomes (50-S and 30-S type). 50-S and 30-S are the large and small subunits of bacteria that colloquially constitute the 70-S type of ribosome. Here, …