Dot blot technique is a method of identifying DNA, RNA and Protein in the sample. It is the simplified form of Southern, Northern and Western blotting for DNA, RNA, and protein isolation, respectively. This method can detect the presence or absence of biomolecule in a single run. Dot blot technique is a prevalent method in …
Cytoplasmic inheritance also termed as maternal inheritance or maternal effect. A cytoplasm comprises some autonomous organelles like mitochondria and plastids that possess their own genetic material. Mitochondria is present in both animal and plant cell, whereas plastids are only found in the plant cell. As we know, nucleic acids like DNA and RNA are responsible …
Taxonomic categories can define as the hierarchical classification of the different individuals into a more specific sequence by categorizing them into seven categories starting from the origin to the organisms. The taxonomic category was first given by the scientist Carl Linnaeus in the 18th century. Linnaeus also developed a system called binomial nomenclature. Linnaean hierarchy, …
Arabinose operon is one of the regulatory systems found in the bacterial cell (E.coli), facilitating arabinose catalysis. L-arabinose operon and ARA-operon are the two alternative names of the arabinose operon. Ara-operon system provides energy to the cell by the breakdown of arabinose into xylulose 5-phosphate. Arabinose is a 5-C sugar or aldopentose that provides energy …
Southern hybridization is the technique, first given by the scientist E. M. Southern in 1975. It is a type of blotting method that involves transferring the DNA from the solid agarose gel to the adsorbent medium like nitrocellulose or nylon filter paper. Southern blotting is done after DNA hybridization. The target DNA is first cleaved …
Colony hybridization is the method first given by the scientists Grinstein and Hogness in the year 1975. This method isolates the specific DNA sequences or genes from the hybrid DNA. Colony hybridization makes the use of the nitrocellulose filter paper. This technique is very much similar to the replica plating. Colony hybridization involves replicas preparation …
The difference between budding and grafting is mainly due to factors like: Scion (a detached living portion of the plant) Classification In budding, a scion is a bud of the closely related plant is inserted into the rootstock of the original plant. In contrast, in the grafting method, a scion is a stem of a closely …
The microbial food spoilage is defined as the contamination of food by the growth and enzymatic activity of microorganisms. Food spoilage is the process, in which the quality of the food deteriorates to some extent that renders food unacceptable for human consumption. Food spoilage occurs due to the microbial attack, enzymatic digestion, chemical degradation, physical …
Isolation of bacteria is a primary method to separate different groups of microorganisms. It is the method that allows us to discriminate different groups of bacteria based on the growth pattern. Different bacteria grow differently on the different nutrient medium, depending on their growth requirements and other factors like temperature, pH, oxygen availability, etc. Isolation …
The qualitative analysis of lipids helps us determine the presence or absence of lipid, depending upon the colour change. Lipids are the organic biomolecules soluble in non-polar solvents like chloroform, ether, acetone etc. and insoluble in water. In nature, different varieties of lipids show structural diversity among each other. Some common lipids are fatty acids, …