ELISA test stands for Enzyme–Linked Immunosorbent Assay. It is a type of serological test and immunoassay technique. In the ELISA test, an enzyme links to the antibodies particularly to detect the presence of proteins like antigens. The ELISA method was evolved from the RIA technique in the 1960s. Therefore, the ELISA technique is more or …
Gram staining is used widely and the most popular method in laboratories. It is the type of differential staining, which makes the use of more than one stains to differentiate the bacteria. The gram staining method was first given in 1884 by the Danish scientist and Physician Han’s Christian Gram. The Gram stain is the differential …
The difference between gram-positive and gram-negative bacteria is mainly due to the following factors like: Cell wall difference Staining difference Cell wall difference: Gram-positive bacteria contain high peptidoglycan content (70-80%) and low lipid content (1-4%). It lacks lipopolysaccharide and porins that are present on the outer membrane, and it has less periplasmic space. Gram-negative bacteria …
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Transduction is a mechanism of genetic recombination that occurs in bacteria where the incorporation of host cell DNA and the bacteriophage genetic material occurs, which results in the formation of recombinant DNA. It was given by scientists in 1952 by Norton Zinder and Joshua Lederberg. Bacterial transduction occurs through generalized and specialized transduction. In generalized …
The differences between the paper and thin layer chromatography are due to the following properties like: The principle for the particle separation Use of stationary phase The main principle behind the particle separation in the paper chromatography is partition type, whereas in thin layer chromatography it is adsorption type. Paper chromatography makes the use of …
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The difference between humoral and cellular immunity are mainly due to two factors like the type of an immune response and the regulation of immunity. Type of Immune response: Humoral immunity produces an antibody-mediated immune response, whereas cellular immunity produces a cell-mediated immune response. Regulation of Immunity: B cell mainly regulates the humoral immunity, whereas …
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Anther culture is a type of tissue culture technique, where a part of a plant (anther) gives rise to an androgenic haploid plant when cultured on a nutrient medium under optimal conditions. The nutrient media provide all the necessary factors that are required for the growth of the cell. The anther culture technique comes under …
ABO blood grouping is based on the principle of an agglutination reaction. It is the popular method for blood group identification to determine the presence and absence of cellular antigens and their relative antibodies in the blood. In blood typing, the detection of antigen in the donor’s RBCs is called forward typing. In contrast, the detection …
Rhizopus is a type of fungus that belongs to the class Zygomycetes, as its species produce zygospore in the sexual reproductive phase. They are also called bread, black or pin mould. These can reproduce by vegetative, asexual and sexual methods through fragmentation, sporangiospores and zygospores formation, respectively. Rhizopus species are cosmopolitan in distribution. It grows …
Aspergillus is a common fungus, which is present everywhere (in the soil, air, water etc.), or we can say it is present in a wide range of conditions. Therefore, these are ubiquitous in nature. It consists of several 100-500 species, among which some are economically important, and some are deleterious, i.e. pathogenic. It was named …