Colony hybridization is the method first given by the scientists Grinstein and Hogness in the year 1975. This method isolates the specific DNA sequences or genes from the hybrid DNA. Colony hybridization makes the use of the nitrocellulose filter paper. This technique is very much similar to the replica plating. Colony hybridization involves replicas preparation …
The difference between budding and grafting is mainly due to factors like: Scion (a detached living portion of the plant) Classification In budding, a scion is a bud of the closely related plant is inserted into the rootstock of the original plant. In contrast, in the grafting method, a scion is a stem of a closely …
The microbial food spoilage is defined as the contamination of food by the growth and enzymatic activity of microorganisms. Food spoilage is the process, in which the quality of the food deteriorates to some extent that renders food unacceptable for human consumption. Food spoilage occurs due to the microbial attack, enzymatic digestion, chemical degradation, physical …
Isolation of bacteria is a primary method to separate different groups of microorganisms. It is the method that allows us to discriminate different groups of bacteria based on the growth pattern. Different bacteria grow differently on the different nutrient medium, depending on their growth requirements and other factors like temperature, pH, oxygen availability, etc. Isolation …
The qualitative analysis of lipids helps us determine the presence or absence of lipid, depending upon the colour change. Lipids are the organic biomolecules soluble in non-polar solvents like chloroform, ether, acetone etc. and insoluble in water. In nature, different varieties of lipids show structural diversity among each other. Some common lipids are fatty acids, …
Mu phage is also called temperate or transposable phage because it causes transposition of the genes into the host cell, at the time of multiplication. It mainly attacks the members or species of enterobacteria family, and that’s why also known as “Enterobacterial phage Mu”. The transposition of Mu-phage occurs by either of the two mechanisms: …
Biohydrogen is now commercially produced as a biofuel. It is the advanced biofuel, which makes the use of living biomass or organisms for the production. Biohydrogen is now considered best among all the biofuels as it accounts to fulfil all the energy demands as it can be obtained from the sustainable resources like: Biological splitting …
The qualitative analysis of amino acids is characterized by the colour change, precipitation, ring formation etc. based on which the amino acids can be detected and classified. The colour change is due to the change in the moiety or the structural configuration, in which the functional group of amino acid react with the specific reagent …
Absorption of water in plants is a vital process that is important for plant growth and other metabolic activities. Water absorption in lower plants takes place by the process of osmosis through the whole plant body. In higher plants, the mechanism of water absorption is through the root hairs. Plants mainly absorb water from the …
The difference between hypertonic and hypotonic solution is mainly due to the factors like: Solute concentration Solvent concentration Effect on a cell A hypertonic solution has a high solute concentration, whereas hypotonic solution has a low solute concentration. The concentration of the solvent is low in the hypertonic solution and high in the hypotonic solution. …
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