Supriya N

Oxidase Test

Oxidase test checks the production of cytochrome-c oxidases, whose presence in the test organism becomes evident by the formation of the blue-purple coloured complex (indophenols). It uses TMPD (tetramethyl phenylenediamine dihydrochloride) reagent. In an oxidized state, TMPD develops a blue-purple coloured complex while remains colourless in a reduced state. Therefore, we can say that the …

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Catalase Test

Catalase test is one of the biochemical analysis that generally comes into use in identifying the organisms (whether they are catalase producers or not). It is primarily used to distinguish between the two classes of gram-positive bacteria, namely Staphylococcus and Streptococcus species. Streptococcus bacteria are mostly facultative anaerobes (may produce catalase), while few are obligate …

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Messenger RNA

Messenger RNA abbreviated as “m-RNA”, and it contains specific codons encoding particular amino acids, which later synthesize proteins (as amino acids are the monomer units of proteins). Protein is an element that is required to develop life forms, so a cell must translate specific proteins. Therefore, for proper growth and cell development, gene translation or …

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Methyl Red Test

Methyl red test differentiates whether a test organism can undergo mixed acid fermentation or not by allocating adequate glucose. Organisms that can ferment glucose will give a positive MR test by producing a large amount of acid, while some organisms give a negative MR test. MR-test also helps to identifying and categorizing the different genera of …

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Indole Test

Indole test is a biochemical test determining and classifying the bacteria based on their tendency to decompose amino acid (Tryptophan) into a heterocyclic organic compound (Indole). It is a part of a biochemical method popularly known as the IMViC test, in which ‘I’ stands for the indole test. Indole is one of the end products …

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Bacterial Conjugation

Bacterial conjugation is a gene transfer mechanism introduced by the scientists named Lederberg and Tatum in 1946. The conjugation method was first studied in Escherichia coli. The formation of a conjugation tube is the characteristic feature of a conjugation mechanism. Besides transduction and transformation mechanisms, some bacteria passes the genetic information from a donor to …

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Mitotic Division

Mitotic division shows a pattern of cell division, where a single cell multiplies into two similar daughter cells by the segregation of newly replicated chromosomes in the nucleus. It is the intermediate phase of the cell division that activates after the G-2 interphase and ends with cytokinesis. Mitosis is a part of the M-phase cell …

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Centrifugation Method

The centrifugation method separates different components or the suspended particles from the homogenous solution through centrifugal force. A natural gravitational force can also concentrate particles but in an indefinite time. To increase particle separation efficiency, a centrifugal force function to separate particles by sedimentation property. It separates particles based on their physical properties like size, …

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