Negative staining or Indirect staining refers to a staining technique that helps us visualize various microorganisms through light and electron microscopy. In bright field microscopy, the method of indirect staining employs liquid medium (black coloured dyes) like Nigrosin and India ink that stains the background, leaving the bacteria unstained. The negative staining technique uses a …
Chlamydia is an obligate intracellular parasite that functions as a “Large virus”. Chlamydias are entirely dependent on the host cells for energy metabolism, like viruses. However, they are true bacteria and differ from viruses by many ways. Like bacteria, chlamydia also possesses both DNA and RNA. They multiply by binary fission, whereas viruses go through …
The difference between selective and differential media is mainly due to the following factors: Intended use of the media: The purpose of both selective and differential media differs. A selective media only permits the development of particular organisms, while a differential media discerns one species of the organism from the others that are growing on …
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MacConkey agar media was first introduced by a scientist named Alfred Theodore MacConkey in the year 1890s. It is primarily exercised to isolate and differentiate the non-fastidious, gram-negative bacilli belonging to the Enterobacteriaceae family. Previously, it was first considered as “Solid differential media”. But now, both liquid broth and solid culture MacConkey media preparations are …
Pinocytosis is a kind of endocytic process. It refers to the cellular mechanism where the bilayer cell membrane invaginates to form a sac to uptake the extracellular fluids and solutes into the cytoplasm. Pinocytosis is a spontaneous endocytic process, which occurs in almost all cells. It prevalently occurs in eukaryotes. Pinocytosis, cell drinking or fluid-phase …
Phagocytosis is a method of endocytosis. It refers to the cellular mechanism where the invagination of the plasma membrane causes internalization of the extracellular contents into the cell in the form of vesicles. Internalization is a process where a plasma membrane undergoes successive invagination to form membrane-bound vesicles, which later detach from the cell membrane …
Exocytosis is a means of membrane transportation that expels the intracellular material out of the cell. The transport of the substance is mediated by the vesicles that eliminate the cell debris and release specific proteins, enzymes, hormones etc., outside the cell. It is a type of active transport mechanism that makes the use of ATP. …
The difference between the cell wall and the cell membrane is primarily due to the differences in three factors like composition, function and permeability. The cell wall composition mainly comprises proteins and carbohydrates, whereas a cell or plasma membrane comprises lipids, proteins, and some amount of carbohydrate. The functional role of the cell wall is to …
A motility test refers to the biochemical or microscopic examination of an organism that checks the existence of cellular motility. By performing this test, we could differentiate the two major groups of bacteria, namely motile and non-motile, based on their cellular movement. Few organisms are motile, and some are non-motile. Still, all living organisms tend …
Vermicomposting is a composting technique, which turns the organic debris into a humus-like product by employing earthworms. “Vermicompost” is the compost produced by the vermicomposting unit. The vermicompost merely refers to the earthworm’s excrement, which provides essential nutrients, aeration, porosity, structure, fertility and water-holding capacity to the soil and plant body. The vermicomposting method requires …