Restriction Enzyme

Restriction enzyme refers to as “Restriction endonuclease” which was discovered during the study of Entero-bacteriophage where the E.coli inhibits the phage activity. In 1978, Werner Arber, Daniel Nathans, Hamilton O Smith won the Nobel Prize for the characterization and discovery of restriction enzyme. Restriction endonuclease also refers to as “Molecular scissors” which are widely used …

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Transfer RNA

Transfer RNA is a type of RNA which acts as the “Intermediary element” which plays a significant role in the loading and transferring of amino acids to the site of protein synthesis, i.e. Ribosome. The t-RNA first decodes the information or the nucleotide sequences carried by the m-RNA. Therefore, t-RNA also plays a major role …

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Difference Between Flagella and Pili

Flagella and pili are the two surface appendages found commonly in prokaryotes. There are some significant differences between the flagella and pili which distinguishes both of them by the following properties like structure, emergence and functional role. Structure: The structure of flagellum is whip-like whereas the structure of pili is thread or hair-like. Emergence: The …

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Difference Between Amphibians and Reptiles

Amphibians and reptiles are the animals which belong to the phylum “Chordata” and are “Cold-blooded animals”. The significant differences between amphibians and reptiles are due to the differences in the properties like life cycle, fertilization and skin type. Amphibians have dual existence in their life cycle, whereby getting birth they adapt themselves to the aquatic …

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Agglutination Reaction

Agglutination Reaction is the immunological reaction where the specific antigen and antibody reaction takes place in vitro.  Agglutination can define as the clumping reaction where clumps or aggregates are formed.  As a result of agglutination, a cross-linked structure forms which refer as “Lattice” that appears in the form of visible aggregates. Agglutination reaction is a sensitive, …

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Precipitation Reaction

A precipitation reaction is a test which is involved in the serology, for the detection of antigens and antibodies. It is the reaction, where the soluble reactants, i.e. antigen and antibody are converted into the aggregated form. The sensitivity of the precipitation reaction is comparatively less than the other immunoassay reactions. For the precipitation reaction, polyclonal …

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