Microbiology

Nucleotide

A nucleotide is a compound, which can form a polynucleotide chain by the union of nitrogenous bases and sugar-phosphate group. Monomers of nucleotide units are connected via a covalent phosphodiester bond. Nitrogenous bases, i.e. purines and pyrimidine, are attached via weak hydrogen bonds. The bases link with a deoxyribose pentose sugar via an N-glycosidic bond. …

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Continuous Culture

The continuous culture is an open cultivation process, in which the cell growth is maintained in the continuous mode of operations. It works as an open cultivation system, in which the fresh nutrient medium is continuously added into the culture vessel rather than recycling and reuse of the nutrients or substrate. There is an inlet pump …

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Transfer RNA

Transfer RNA is an RNA type that acts as the intermediary element, and plays a significant role in the loading and transferring amino acids to the site of protein synthesis, i.e. Ribosome. The t-RNA first decodes the information or the nucleotide sequences carried by the m-RNA. Therefore, t-RNA also plays a major role in translating …

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Agglutination Reaction

Agglutination reaction is an immunological assay, which results in the specific antigen and antibody reaction in vitro. Agglutination purely refers to the clumping or aggregation of the particles. As a result of agglutination, a cross-linked structure or lattice appears in the form of visible aggregates. Agglutination reaction is a sensitive, easy and cost-effective method to carry …

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Precipitation Reaction

A precipitation reaction is a test involved in the serology, for the qualitative and quantitative detection of antigens and antibodies. It is a reaction, where the soluble reactants (antigen and antibody) convert into an aggregated form. The sensitivity of the precipitation reaction is comparatively less than the other immunoassay reactions. For the precipitation reaction, polyclonal …

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